Acute Eye Irritation and Corrosiveness Test

Our company offers an acute eye irritation/corrosion test to detect primary irritation and corrosion of the eyes and mucous membranes of laboratory animals and to provide information on the potential hazards of the human eye and mucous membrane exposure to the subject.

Test Purpose

1. To assess the toxicity of the test substance to experimental animals and to provide a basis for acute toxicity classification.
2. To provide a preliminary understanding of dermal absorption and the mode of toxic action of the test substance.
3. To provide a basis for the selection of doses for other toxicological tests.
4. Facilitate labeling management.

Test Range

Veterinary drugs, pesticides, chemicals, fungicides, organic toxicants, solid waste, domestic waste leachate, hazardous waste, sewage, sludge, water quality, surfactants, etc.

About Acute Eye Irritation and Corrosiveness Test Service

Test Method

The sample was dropped in a single dose into the conjunctival sac of one eye of each experimental animal, and the untreated other eye was used as its own control.

The irritation effect of the test substance on the eye is evaluated by observing the degree of irritation and corrosive effect on the eye of the animal for a specified time interval. The observation period should be sufficient to evaluate the reversibility and irreversibility of the irritation effect.

If the change is reversible for verification, the irritation effect is irritation. If the tissue damage is irreversible, the corrosive effect is corrosion.

Subjects

• Liquid samples generally do not need to be diluted and can be used directly from the stock solution.
• Solid or granular samples should be ground to a fine powder and well moistened with water.
• For aerosol samples, the rabbit's eye should be propped open and the aerosol spray should be pressed against the rabbit's eye for 1 s at a distance of 10 cm directly in front of the eye.
• If the sample is a strong acid or a strong base (pH≤2 or ≥11.5), or if it has been proven to be corrosive or highly irritating to the skin, the eye irritation test can no longer be performed.

Test Animals

Our company preferred healthy adult white rabbits, at least three of which were used. We examined both eyes of the test animals within 24 h prior to the start of the test (including examination with sodium fluorescein). Animals with signs of eye irritation, corneal defects and conjunctival damage were not used for the test.

Observation Index

The eyes of the animals were examined at 1 h, 24 h, 48 h, 72 h, and on days 4 and 7 after dropping the test substance. Effects on the cornea, iris, conjunctiva, and other damage were recorded and reported.

Acute Eye Irritation and Corrosiveness Test Report

The final test report we provide includes the following.

• Sample acceptance date and sealing samples.
• Test start and end date, test project leader, technical leader of the test unit.
• Test summary.
• Sample name, the active ingredient, purity, dosage form, physical and chemical properties, and preparation method.
• Experimental animal species, strain, level, number, weight, sex, source, quarantine, adaptation. Laboratory animal feeding environment, including temperature, relative humidity, serving materials, and laboratory animal facilities use permit number.
• Test conditions and methods, including the main instruments and equipment, the amount of poisoning, the time and method of poisoning, etc.
• Test results: Summarized in textual descriptions and tables item by item.
• Test conclusion: Give a clear conclusion on the intensity of eye irritation and clarify whether it is corrosive or not.
• Description of the original record keeping.

If you are looking for the best solution in the field of toxicology research, please feel free to contact us.

It should be noted that our service is only used for research, not for clinical use.